Prestained Protein Ladder - Broad molecular weight (10 (2024)

Overview

• Product name

  Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)
  See all Prestained Protein Ladder reagents

  See Also

  Protein Standards & Ladders - USPrestained Protein Ladder - Mid-range molecular weight (10

• Tested applications

  Suitable for: SDS-PAGE, WBmore details

• General notes

  Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

  This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

  Key product features:

  • Broad range: 10-245 kDa
  • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
  • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
  • Sharp bands
  • Recommended loading: ~1.5 - 2.5µl

  Review other protein ladders in theunstained and prestained protein ladder guide.

  This product was previously calledPrism Ultra Protein Ladder (10-245 kDa).

  Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Properties

• Form

  Liquid

• Storage instructions

  Please see notes section.

• Storage buffer

  pH: 7.5
  Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0.02% DTT, 15% Glycerol

• Concentration information loading...

• Research areas

  • Kits/ Lysates/ Other
  • Tools and Reagents
  • Western Blot Tools and Reagents
  • Protein ladders

• Alternative names

  • Molecular weight marker
  • Prism protein ladder
  • SDS PAGE protein ladder prestained

  see all

• SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)

  Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

• SDS download

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• Datasheet download

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ab116028 has been referenced in 16 publications.

• Thejer BM et al. PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. BMC Mol Cell Biol 21:24 (2020). PubMed: 32245408
• Depoix CL et al. Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts. Invest New Drugs 38:547-557 (2020). PubMed: 31155684
• Negri S et al. Group 1 metabotropic glutamate receptors trigger glutamate-induced intracellular Ca2+ signals and nitric oxide release in human brain microvascular endothelial cells. Cell Mol Life Sci 77:2235-2253 (2020). PubMed: 31473770
• Chowdhury P et al. Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor. Infect Genet Evol 85:104418 (2020). PubMed: 32561295
• Ghoshal S et al. Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India. Malar J 19:367 (2020). PubMed: 33054833

View all Publications for this product