Anti-Collagen I antibody [COL-1] (ab6308) (2024)

Overview

• Product name

  Anti-Collagen I antibody [COL-1]
  See all Collagen I primary antibodies

• Description

  Mouse monoclonal [COL-1] to Collagen I

• Host species

  Mouse

• Tested applications

  Suitable for: IHC-Fr, WBmore details

• Species reactivity

  Reacts with: Human
  Predicted to work with: Rat, Cow, Pig, Deer

• Immunogen

  Full length native protein (purified) corresponding to Cow Collagen I. Cow skin collagen type I

• Epitope

  The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.

• Positive control

  • WB: Natural Cow Collagen I protein (ab7526), IHC-Fr: Human tonsil tissue.

• General notes

  Production of this antibody has been changed on 23^rd June 2016. The following lots are from ascites and are still in stock as of 23^rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  See Also

  Prestained Protein Ladder - Broad molecular weight (10Multicolour protein ladder, broad range, Spectra™Spectra™ Multicolor Broad Range Protein Ladder

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• Storage buffer

  pH: 7.40
  Preservative: 0.0976% Sodium azide
  Constituent: PBS

• Concentration information loading...

• Purity

  Proprietary Purification

• Purification notes

  Purified from Tissue culture supernatant.

• Clonality

  Monoclonal

• Clone number

  COL-1

• Isotype

  IgG1

• Research areas

  • Cell Type Markers
  • Tumor Associated

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Proteins
  • Collagen

  • Stem Cells
  • Mesenchymal Stem Cells
  • Surface Molecules

  • Stem Cells
  • Mesenchymal Stem Cells
  • Osteogenesis

  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

Associated products

• Compatible Secondaries

• Isotype control

  • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

• Positive Controls

  • Native Cow Collagen I protein (ab7526)
  • Native Human Collagen I protein (ab7533)

• Recombinant Protein

  • Recombinant Human Collagen I protein (ab158152)

• Related Products

  • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
  • Mouse Pro-Collagen I alpha 1 ELISA Kit (ab210579)
  • Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
  • Mouse Pro-Collagen I alpha 1 Matched Antibody Pair Kit (ab216791)
  • 10x Citrate Buffer pH 6.0 (ab64214)

Applications

Target

• Function

  Type I collagen is a member of group I collagen (fibrillar forming collagen).

• Tissue specificity

  Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

• Involvement in disease

  Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
  Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
  Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
  Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
  Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
  Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.

• Sequence similarities

  Belongs to the fibrillar collagen family.
  Contains 1 fibrillar collagen NC1 domain.
  Contains 1 VWFC domain.

• Post-translational
  modifications

  Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
  O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

• Cellular localization

  Secreted > extracellular space > extracellular matrix.

• Information by UniProt

• Database links

  • Entrez Gene: 282187 Cow
  • Entrez Gene: 282188 Cow
  • Entrez Gene: 1277 Human
  • Entrez Gene: 1278 Human
  • Entrez Gene: 29393 Rat
  • Entrez Gene: 84352 Rat
  • Omim: 120150 Human
  • Omim: 120160 Human

  see all

• Alternative names

  • Alpha 1 type I collagen antibody
  • Alpha 2 type I collagen antibody
  • alpha 2 type I procollagen antibody

  see all

Images

Protocols

Datasheets and documents

References (464)